Glossary of Medical Terms

Our online medical glossary of medical terms and definitions includes definitions for terms related to treatment, and general medicine

COLONY HYBRIDISATION

A genetics lab technique used to designate which colonies of bacteria on an agar plate contain a special sequence of DNA or a special gene. The technique involves pressing a nylon or nitrocellulose membrane onto the plate so that every colony contributes a little smudge of itself to the membrane, then treating the membrane with chemicals and heat, then washing the membrane with a labelled probe to find the specific DNA sequence. The smudges which are indicated by the probe are then compared back to the colonies on the agar plate. This technique is often used in conjunction with experiments inclusion the making of genomic libraries.
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