OVERLAP HYBRIDIZATION

A procedure to find and sequence a gene whose approximate position in a chromosome is known by classical genetic linkage research. Starting with the known sequence of a gene shown by classical genetics to be around to the novel gene, new clones are picked from a genomic library by hybridisation with a short probe generated from the appropriate end of the known sequence. The new clones are then sequenced, new probes generated and the process repeated before the gene of interest is reached. This entry appears with alow from the Vocabulary of Cell and Molecular Biology